Using the FORESTS and KEGG databases to investigate the metabolic network of Eucalyptus

In this work we apply a bioinformatics approach to determine the most important enzymes of the metabolic network of Eucalyptus to determine the coverage of the genome in the FORESTS library. We conclude that the library does not cover completely the metabolism of the organism. However, some important pathways could be analyzed, especially the lignin synthesis. We found that four of the most important enzymes predicted are involved in this pathway

Multigene families encode the major enzymes of antioxidant metabolism in Eucalyptus grandis L

Antioxidant metabolism protects cells from oxidative damage caused by reactive oxygen species (ROS). In plants, several enzymes act jointly to maintain redox homeostasis. Moreover, isoform diversity contributes to the fine tuning necessary for plant responses to both exogenous and endogenous signals influencing antioxidant metabolism. This study aimed to provide a comprehensive view of the major classes of antioxidant enzymes in the woody species Eucalyptus grandis. A careful survey of the FORESTs data bank revealed 36 clusters as encoding antioxidant enzymes: six clusters encoding ascorbate peroxidase (APx) isozymes, three catalase (CAT) proteins, three dehydroascorbate reductase (DHAR), two glutathione reductase (GR) isozymes, four monodehydroascorbate reductase (MDHAR), six phospholipid hydroperoxide glutathione peroxidases (PhGPx), and 12 encoding superoxide dismutases (SOD) isozymes. Phylogenetic analysis demonstrated that all clusters (identified herein) grouped with previously characterized antioxidant enzymes, corroborating the analysis performed. With respect to enzymes involved in the ascorbate-glutathione cycle, both cytosolic and chloroplastic isoforms were putatively identified. These sequences were widely distributed among the different ESTs libraries indicating a broad gene expression pattern. Overall, the data indicate the importance of antioxidant metabolism in eucalyptus

Crescimento rápido da cromomicose (Fonsecae pedrosoi) utilizando eucalipto (Eucalyptus SP) como meio de cultivo / Fast growth of chromomycosis (Fonsecae pedrosoi) using eucalyptus (Eucalyptus SP) as culture medium

Descreve-se o crescimento do fungo Fonsecae pedrosoi em um fragmento de Eucalyptus e comparam com o crescimento no meio Saboraud dextrose ágar. O material para o cultivo foi obtido de uma lesäo de aspecto verrucoso de 01 (um) paciente internado no Hospital "Lauro de Souza Lima" de Bauru-SP